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ASTM F3088 : 14

Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions

Standard Details

1.1 This test method covers a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments. This approach measures the force required to detach cells from a substrate. Adhesion, among many variables, may vary due to changes in the phenotype of the cells.

1.2 This test method does not cover methods to verify the uniformity of coating of surfaces, nor does it cover methods for characterizing surfaces.

1.3 The cells may include adult, progenitor or stem cells from any species. The types of cells may include chondrocytes, fibroblasts, osteoblast, islet cells, or other relevant adherent cell types.

1.4 This test method does not cover methods for isolating or harvesting of cells. This test method does not cover test methods to quantitate changes in gene expression, or changes in biomarker type or concentration, as identified by immunostaining. Nor does this test method cover quantitative image analysis techniques.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status : Historical
Standard Type: Main
Document No: ASTM F3088 : 14
Document Year: 2014
Pages: 7
  • Section Volume:
  • 13.02 Volume 13.02 Medical and Surgical Materials and Devices (II): F2502-Latest; Emergency Medical Services; Search and Rescue; Anesthetic and Respiratory Equipment
  • ICS:
  • 11.100.99 Other standards related to laboratory medicine

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ASTM F3088 : 14
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