ASTM E1366 : 23

Standard Practice for Standardized Aquatic Microcosms: Fresh Water

Standard Details

1.1 This practice covers procedures for obtaining data concerning toxicity and other effects of a test material to a multi-trophic level freshwater community, independent of the location of the test.

1.2 These procedures also might be useful for studying the fate of test materials and transformation products, although modifications and additional analytical procedures might be necessary.

1.3 Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting multi-trophic level tests.

1.4 This practice is arranged as follows:

	Section

Referenced Documents	2
Terminology	3
Summary of Practice	4
Significance and Use	5
Apparatus	6
Facilities	6.1
Container	6.2
Equipment	6.3
Hazards	7
Microcosm Components	8
Medium	8.1
Medium Preparation	8.2
Sediment	8.3
Microcosm Assembly	8.4
Test Material	9
General	9.1
Stock Solution	9.2
Nutrient Control	9.3
Test Organisms	10
Algae	10.1
Animals	10.2
Specificity of Organisms	10.3
Sources	10.4
Algal Culture Maintenance	10.5
Animal Culture Maintenance	10.6
Procedure	11
Experimental Design	11.1
Inoculation	11.2
Culling	11.3
Addition of Test Material	11.4
Measurements	11.5
Reinoculations	11.6
Analytical Methodology	12
Data Processing	13
Calculations of Variables from Measurements	14
Statistical Analyses	15
Acceptability of Test	16
Interpretation of Results	17
Report	18
Annex	Annex A1
Appendices
Relationship of Media	Appendix X1
Statistical Guidance	Appendix X2

1.5 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.Specific hazard statements are given in Section 7.

1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International StandardDetails, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.