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Oilseeds — Extraction of oil and preparation of methyl esters of triglyceride fatty acids for analysis by gas chromatography (rapid method)
This document is applicable to the following oilseeds: rape and mustard with low erucic acid content (< 2 %), sunflower, soya beans, linseed.
NOTE Applying this rapid method to high erucic acid content rapeseed leads to an overestimation of erucic acid content by approximately a mass fraction of 1 %. This difference was observed in Reference [6] and could be due to the partial extraction of the oil from the sample (yield around 70 %). High content of erucic acid in triglycerides could increase their solubility in hexane because of the lipophilic effect of the carbon long-chain (C22). However, as this effect was not checked on a large set of high erucic rapeseed samples, it is not appropriate to apply a correction factor to the erucic acid content when analysing high erucic acid rapeseed.
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