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Microbiology of food and animal feeding stuffs — Horizontal methods for the detection and enumeration of Enterobacteriaceae — Part 1: Detection and enumeration by MPN technique with pre-enrichment
Enumeration is carried out by calculation of the most probable number (MPN) after incubation at 37 °C in liquid medium.
This method is applicable when the microorganisms sought are expected to need resuscitation before enrichment, and when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.
A limitation on the applicability of ISO 21528-1:2004 is imposed by the susceptibility of the method to a large degree of variability
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