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ISO 21446:2019

Infant formula and adult nutritionals — Determination of trans and total (cis + trans) vitamin K1 content — Normal phase HPLC

Standard Details

This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for StandardDetails and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.

General Information

Status : Published
Standard Type: Main
Document No: ISO 21446:2019
Document Year: 2019
Pages: 20
Edition: 1
  • ICS:
  • 67.050 General methods of tests and analysis for food products

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ISO 21446:2019
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